#3 Biofuels


Learning from Nature, from genes to ecosystems

Module 3 Plant biomass degradation (Biofuels)

Core Concept
Plant cell walls contain a mixture of recalcitrant polysaccharides. Nature has devised many strategies of obtaining energy from these compounds.

Recalcitrant polysaccharides carbohydrates (starch and cellulose) consisting of lots of monosaccharides that joined together by chemical bonds and are difficult to break.

Photosynthesis- The process by which plants, algae, and some bacteria use energy from the sun, water, and carbon dioxide to make glucose. The glucose made through this process is linked together to form starch and cellulose.


Overview and Key terms
Most of the energy used by all living cells comes from the sun. Plants are called autotrophic, because they can make their own food. They do through a process called photosynthesis.
     Photosynthesis-the process used by plants, algae, and some bacteria to capture sunlight energy
     only about 1% of the available energy in sunlight is captured
If you look at a plant cell under the microscope, you can see the chloroplasts.
Chloroplasts up close. (http://www.youtube.com/watch?v=LTglday5zak)
 Chloroplasts contain the following:

thylakoids- the internal membranes
Grana- thylakoids are stacked together in columns
Stroma- semiliquid substance that surrounds the thylakoids
photosystems- embedded in the thylakoid membrane contain pigments
Chlorophyll-primary pigment molecule in most photosystems is the pigments act as an antenna to capture energy from sunlight individual chlorophyll pigments pass the captured energy between them through eh electron transport system

Photosynthesis takes places in three stages
·         capturing energy from sunlight
·         using the captured energy to produce ATP and NADPH
·         using the ATP and NADPH to make carbohydrates from CO2 in the atmosphere  (carbon fixing)
Photosynthesis makes two very important compounds
·         Oxygen
·         Glucose

      The light-dependent reactions in plants and algae use two photosystems
     Photosystem II
      captures a photon of light and releases an excited electron to the electron transport system (ETS)
      the ETS then produces ATP
     Photosystem I
      absorbs another photon of light and releases an excited electron to another ETS
      this ETS produces NADPH
      Fixing Carbon
      The synthesis of organic molecules from CO2 uses the Calvin cycle
     these reactions are also known as C3 photosynthesis
     The Calvin cycle must “turn” 6 times in order to form a new glucose molecule
     the products of the light-dependent reactions are used
      ATP energy drives the cycle
      NADPH provides hydrogen atoms
The energy for living is obtained by breaking down the organic molecules originally produced in plants or other organisms.
How some animals use plants to obtain energy:
The Leaf Cutter Ants, Cows, and Biofuels
The Leaf Cutter Ants


Picture from flickr.com

Leaf-cutter ants use fresh leaf material to grow fungal gardens on. Mature colonies can have over 5 million workers.  They are considered dominant herbivores in Neotropical terrestrial Ecosystems.  Mature colonies have a 10-15 year lifespan and can harvest  about 400kg of dry weight (or 1600-4000 kg of wet weight) plant material per year.
The fungal cultivar is unable to metabolize all components of the plant material. Bacterial symbionts are in the fungal gardens that help break down the plant material. However, there are still a lot of recalcitrant polysaccharides found in the material that the ants dump. The ants dump is composed of undigested plant material and old fungal matter. An individual colony can produce about 200kg of refuse per year. These ants dumps are an important source of nutrients in the ecosystem. Dump material accumulates during the dry season and then undergoes rapid decomposition during the rainy season. Some scientists have called these dumps “Nutrient Islands” and many studies show there is a significant increase in the fine-root systems of plants found associated with/near the dumps. In a very similar process as to a compost pile, bacteria found in these dumps help break the chemical bonds of the recalcitrant polysaccharides and make the nutrients available to plants and other organisms.





Cattle


Picture from omafra.gov.on.ca and icanhasinternets.com
Cattle are large domesticated ungulates. Cattle are raised as livestock for meat, as dairy animals (milk, cheese, and ice cream), and as work animals (pulling farm equipment). Other products that are made from cattle include: leather, dung material for fertilizers and fuel. In India and other countries, cattle are sacred. There are almost 2 billion cows in the world today. And in 2009, cattle became the first livestock animal to have its genome mapped.
Cows have one stomach with four compartments and are considered to be ruminants. Ruminants are animals that have a digestive system that allows them to utilize use recalcitrant polysaccharides through a process of regurgitating and rechewing the material "cud". Cud is then reswallowed and digested by symbiotic microorganisms (fungi and bacteria) in the rumen. These microbes are primarily responsible for decomposing cellulose and other carbohydrates into volatile fatty acids that cattle can then use as their source nutrients. After the microbes have completed the process of breaking down the plant material, the cow then digests them and obtains a scource of protein that can be made into milk by the cow.  The way ruminant’s digestive system works and their association with microbes allow them to survive on diet consistenting of grasses and other vegetation.

Overview of Cows  (http://www.youtube.com/watch?v=cWg1u1bUKNc)



How humans are using microbes to create biofules:
Biomass(http://www.nsf.gov/news/special_reports/greenrevolution/)-Choose video Biomass

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